Reviews Dna Sequencing

Edvotek 225 Usage of Restriction Enzymes in DNA Fingerprinting Analysis, For 6 Gels

  • Quick recovery of ultra-pure DNA from agarose gels.
  • Column design permits DNA elution at high concentrations into minimal volumes.
  • Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
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Zymo Research D4007 Zymoclean Gel DNA Recovery Kit with Capped Column, 50 Preps

  • Clean and concentrate (e.g., PCR purification) up to 25 µg DNA in as little as 25µl elution in 2 minutes with 0µl wash residue carryover.
  • Column design allows DNA to be eluted at high concentrations into minimal volumes as little as 25ul.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
  • DNA Recovery: As much as 25ug. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%..
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dNTP Mix P03-CR04-10, 10mM, 1ML

  • dATP, dGTP, dCTP and dTTP 100mM stock solutions are also available with us..
  • Applications PCR amplification of DNA fragments DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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PfuTurbo DNA Polymerase Alternative Detergent

  • dATP, dCTP and dTTP also available with us..
  • Applications PCR amplification of DNA fragments DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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dATP100mM, 0.25ML

  • dATP, dGTP and dTTP also available with us..
  • Applications PCR amplification of DNA fragments DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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dATP, dGTP, dTTP, dCTP 100mM Each, 4 x 0.25 ML

  • Box contains contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
  • Stable at room temperature. Long term storage at -20^oC required..
  • Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR..
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HotStart 2xTaq DNA Pol Master Mix,1ML, P03-CR03SM-1ML

  • Contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
  • HotStart PCR for specific amplification. Comparable with the Platinum Taq polymerase. Good for TA cloning..
  • Dye Minus HotSatrt 2X Master Mix is also available upon request..
  • Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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Genomic DNA Extraction Kit (Bacteria), 50 Preps, 10644 -from Cepham Life Sciences

  • Isolate genomic DNA from both Gram-positive & Gram-negative bacteria.
  • High yield, high quality DNA for sensitive downstream applications including sequencing, PCR & qPCR.
  • Spin columns-collection tubes- 2 mL (50/bag).
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5-Color Set, 2 oz. Each of G,Y,BK,R,BL - Plastic

  • Storage Buffer 20mM Tris-HCl, 1mM DTT, 0.1mM EDTA,100mM KCl 50% glycerol, 0.5%NP40, 0.5%TW20.
  • 10×PCR Buffer 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4, 15mM MgCl2, 0.5% NP-40.
  • Unit definition One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions..
  • Applications PCR amplification of DNA fragments up to 5 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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IBI Scientific IB47010 Gel/PCR DNA Fragment Extraction Sample Kit for 4 Preparations

  • Kit contains (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer.
  • Sample: up to 300mg agarose gel slice and up to 100 microliter PCR product or other enzymatic reaction.
  • expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up.
  • Applications: PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation.
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