Best 12 Metal chelate Chromatography Kit Reviews & Buying Guide

Introducing our Metal Chelate Chromatography Kit - designed to support the efficient purification of histidine-tagged recombinant proteins. By using an immobilized metal affinity chromatography (IMAC) approach, this kit is engineered to capture histidine-tagged proteins with high specificity, purity, and yield.
The Metal Chelate Chromatography Kit streamlines the chromatography process by providing pre-packed columns, buffers, and reagents that are optimized for the IMAC purification of his-tagged proteins. This kit is compatible with various sample matrices, including bacterial lysates and column fractions, and can be used in both manual and automated chromatography workflows.
With our Metal Chelate Chromatography Kit, you can expect outstanding protein binding capacity, high-resolution separation, and reproducible results. The kit's high-quality components deliver efficient purification of your target protein with reduced nonspecific binding.
Explore the Metal Chelate Chromatography Kit today and purify your histidine-tagged protein with ease.
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Metal chelate Chromatography Kit

Metal chelate chromatography is a technique used in protein purification that utilizes a metal ion immobilized on a solid support to bind proteins containing metal-binding regions such as histidine residues. This technique has become increasingly popular due to its high specificity, versatility, and scalability. In this post, we will review the top 12 metal chelate chromatography kits and provide a buying guide to help you select the best kit for your needs.

1. GE Healthcare HisTrap™ FF Crude

This kit is designed for the purification of histidine-tagged proteins directly from crude samples. It utilizes prepacked columns containing nickel-charged resin for high specificity and fast purification. The resin is also reusable and compatible with most commonly used buffers.

2. Thermo Scientific® HisPur™ Ni-NTA Resin

This kit consists of a resin made of agarose beads with a high density of immobilized nickel ions. It offers high binding capacity, low nonspecific binding, and excellent elution efficiency. It is also compatible with multiple expression systems and sample types.

3. Agilent CaptivA PriHis Ni-NTA Resin

This kit features a resin with a high density of immobilized nickel ions, combined with optimized protocols for high purity and yield. Its unique cross-linked agarose beads provide high chemical and mechanical stability, offering a longer lifespan and better reproducibility.

4. Bio-Rad Profinity IMAC Ni-Charged Resin

This kit contains an immobilized nickel resin with a unique binding mechanism that minimizes nonspecific binding and enhances selectivity. It also provides a fast protocol with a one-step purification process, making it suitable for high-throughput applications.

5. Expedeon AbPure™ Ni-NTA Protein Purification Kit

This kit is designed for the purification of histidine-tagged proteins from various sources, including bacteria, mammalian cells, and insect cells. It utilizes prepacked columns with nickel-charged resin and offers high purity and yield with minimal sample loss.

6. Sigma-Aldrich HisPur™ Cobalt Resin

This kit features a resin made of agarose beads with a high density of immobilized cobalt ions. Cobalt ions have a higher affinity for histidine residues than nickel ions, which allows for better selectivity and enhanced purity. Its highly stable resin also allows for multiple use and reproducibility.

7. Merck His GraviTrap™ TALON® Purification Kit

This kit utilizes TALON® metal affinity resin for high specificity and versatility in the purification of histidine-tagged proteins. It features prepacked columns for fast and easy purification, and the reusable resin allows for cost-effective use.

8. Kedrion Biochrom Cu-IMAC Purification Kit

This kit contains a resin made of silica beads with copper ions immobilized on the surface. Copper ions offer a unique selectivity advantage, especially for histidine-tagged proteins with an exposed cysteine residue. It provides a fast and simple isolation protocol with high purity and yield.

9. Cube Biotech His SpinTrap™ Kit

This kit is designed for the purification of histidine-tagged proteins using spin columns and resin with a high density of nickel ions. It provides a simple and fast protocol that can yield high purity and yield. Its spin columns allow for multiple sample processing, making it suitable for small-scale protein analysis.

10. Chromotek GFP-Trap® Kit

This kit utilizes an immobilized GFP-specific nanobody for the purification of GFP-tagged proteins. It features high specificity and affinity, resulting in a low background and improved purity. This kit is also compatible with a variety of species and sample types, making it versatile and user-friendly.

11. Lucigen CaptureSelect™ HIS Affinity Matrix

This kit features a resin with a high binding capacity for histidine-tagged proteins and minimal nonspecific binding. It offers low cost and is compatible with most commonly used buffers, allowing for easy integration into existing protein purification protocols.

12. Abcam His-tag Kit

This kit provides a complete set of reagents and protocol for the purification of histidine-tagged proteins from bacterial and mammalian expression systems. It utilizes prepacked columns with nickel resin and provides high yield and purity with low sample loss.

Buying Guide:

When selecting a metal chelate chromatography kit, consider the following factors:

1. Binding specificity: Ensure that the kit offers binding specificity for your target protein, as well as minimized nonspecific binding.

2. Resin capacity and stability: Check the resin's binding capacity and stability to ensure that it provides efficient and consistent purification performance.

3. Compatibility: Consider the compatibility of the kit with your sample type and expression system to ensure optimal performance.

4. Purification yield and purity: Check the kit's yield and purity specifications to ensure that it provides adequate protein recovery and high purity.

5. Ease of use and scalability: Consider the kit's ease of use, including prepacked columns and simple protocols, as well as scalability to allow for high-throughput processing.


Metal chelate chromatography is a highly efficient and specific technique for protein purification that offers excellent versatility and scalability. The selection of a suitable kit for your needs requires careful consideration of binding specificity, resin capacity, compatibility, yield, purity, ease of use, and scalability. We hope that this review of the top 12 metal chelate chromatography kits and buying guide will assist you in selecting the best kit for your protein purification needs.